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wash buffer  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology wash buffer
    Wash Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fcm+wash+buffer/FCM+Wash+buffer/pm36769335-259-33-44
    Average 93 stars, based on 22 article reviews
    wash buffer - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    other:

    Article Title: INTERFERON-ALPHA MEDIATES THE DEVELOPMENT OF AUTOIMMUNITY BOTH BY DIRECT TISSUE TOXICITY AND THROUGH IMMUNE-CELL RECRUITMENT MECHANISMS
    Article Snippet: Phycoerythrin (PE)-conjugated goat anti-mouse IgG antibody, normal mouse IgG1 and FCM wash buffer were purchased from Santa Cruz (Santa Cruz, CA).

    Blocking Assay:

    Article Title: Anti-invasive and anti-adhesive activities of a recombinant disintegrin, r-viridistatin 2, derived from the Prairie rattlesnake ( Crotalus viridis viridis )
    Article Snippet: .. After blocking, cells were washed with 1 mL 1× PBS, centrifuged, and resuspended in FCM Wash Buffer (Santa Cruz Biotechnology) to 10 6 cells/mL. ..

    Article Title: Functional analysis of a recombinant PIII-SVMP, GST-acocostatin; an apoptotic inducer of HUVEC and HeLa, but not SK-Mel-28 cells
    Article Snippet: .. After blocking, cells were washed with 1mL 1X PBS, centrifuged, and resuspended in FCM Wash Buffer (Santa Cruz Biotechnology) to 10 6 cells/mL. ..

    Article Title: Functional characterization of six aspartate (D) recombinant mojastin mutants (r-Moj): a second aspartate amino acid carboxyl to the RGD in r-Moj-D_ peptides is not sufficient to induce apoptosis of SK-Mel-28 cells
    Article Snippet: .. After blocking, cells were washed with 1mL 1× PBS, centrifuged, and resuspended in FCM Wash Buffer (Santa Cruz Biotechnology) to 10 6 cells/mL. ..

    Incubation:

    Article Title: Induction of autophagy, apoptosis and aquisition of resistance in response to piceatannol toxicity in MOLT-4 human leukemia cells.
    Article Snippet: Accepted Manuscript Induction of autophagy, apoptosis and aquisition of resistance in response to piceatannol toxicity in MOLT-4 human leukemia cells Kamila Siedlecka-Kroplewska, Tomasz Ślebioda, Zbigniew Kmieć PII: S0887-2333(19)30002-5 DOI: https://doi.org/10.1016/j.tiv.2019.03.040 Reference: TIV 4508 To appear in: Toxicology in Vitro Received date: 21 January 2019 Revised date: 9 March 2019 Accepted date: 29 March 2019 Please cite this article as: K. Siedlecka-Kroplewska, T. Ślebioda and Z. Kmieć, Induction of autophagy, apoptosis and aquisition of resistance in response to piceatannol toxicity in MOLT-4 human leukemia cells, Toxicology in Vitro, https://doi.org/10.1016/ j.tiv.2019.03.040 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.



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    Santa Cruz Biotechnology flow cytometry buffer
    Surface Gal3 defines a subtype of epithelial CSC. ( a ) Colorectal cancer cells LiM6 and DLD1 were investigated by flow <t>cytometry</t> for cell surface markers CD24/CD44 (left). CD24+/CD44+-cells (in black) were then investigated for CD166/EpCAM-expression (middle) and CD24+/CD44+/CD166+/EpCAM+-positive cells (in black middle) for Gal3-expression (right panel), where ++++ Gal3 − in green refers to cells positive for CD24, CD44, CD166, and EpCAM, but negative for Gal3, and ++++ Gal3 + in red refers to cells positive for CD24, CD44, CD166, EpCAM, and Gal3. ( b ) Pancreatic cancer cell lines AsPC1 and L3.6pl were subjected to the same analysis as in ( a )
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    Image Search Results


    Surface Gal3 defines a subtype of epithelial CSC. ( a ) Colorectal cancer cells LiM6 and DLD1 were investigated by flow cytometry for cell surface markers CD24/CD44 (left). CD24+/CD44+-cells (in black) were then investigated for CD166/EpCAM-expression (middle) and CD24+/CD44+/CD166+/EpCAM+-positive cells (in black middle) for Gal3-expression (right panel), where ++++ Gal3 − in green refers to cells positive for CD24, CD44, CD166, and EpCAM, but negative for Gal3, and ++++ Gal3 + in red refers to cells positive for CD24, CD44, CD166, EpCAM, and Gal3. ( b ) Pancreatic cancer cell lines AsPC1 and L3.6pl were subjected to the same analysis as in ( a )

    Journal: Cell Death & Disease

    Article Title: Cell surface galectin-3 defines a subset of chemoresistant gastrointestinal tumor-initiating cancer cells with heightened stem cell characteristics

    doi: 10.1038/cddis.2016.239

    Figure Lengend Snippet: Surface Gal3 defines a subtype of epithelial CSC. ( a ) Colorectal cancer cells LiM6 and DLD1 were investigated by flow cytometry for cell surface markers CD24/CD44 (left). CD24+/CD44+-cells (in black) were then investigated for CD166/EpCAM-expression (middle) and CD24+/CD44+/CD166+/EpCAM+-positive cells (in black middle) for Gal3-expression (right panel), where ++++ Gal3 − in green refers to cells positive for CD24, CD44, CD166, and EpCAM, but negative for Gal3, and ++++ Gal3 + in red refers to cells positive for CD24, CD44, CD166, EpCAM, and Gal3. ( b ) Pancreatic cancer cell lines AsPC1 and L3.6pl were subjected to the same analysis as in ( a )

    Article Snippet: To identify and isolate CSC, parental cells were collected in Versene solution (Invitrogen, Carlsbad, CA, USA, cat#15040), washed and blocked with flow cytometry buffer (Santa Cruz Biotechnology, Dallas, TX, USA), labeled with the following primary conjugated antibodies and their matched isotypes: anti-CD24-PE-Cy7(561646), anti-EpCAM-FITC (BD Bioscience, San Jose, CA, USA, 347197) anti-CD44-APC-eFluor 780(47044182), anti-CD166-PerCPeFluor 710(46166842), anti Gal3-PE(12-5301-83) (all from - eBioscience, San Diego, CA, USA).

    Techniques: Flow Cytometry, Expressing

    Silencing of Gal3 shifts CSC subset to a Gal3 negative CSC subset. ( a ) Western blot analysis for comparison of total Gal3 in LiM6TR or DLD1TR colon cancer cells and L3.6pl or AsPC1 pancreatic cancer cells after infection with lentiviral particles for control-shRNA ( c ) or Gal3-shRNA (G). Each pair of control and Gal3 knockdown cells were run side by side on the same gel. Comparison of Gal3 expression between cell lines has been previously published ( b ) Flow cytometric analysis of colon cancer and pancreatic cancer cells after infection with lentiviral particles for control-shRNA (red trace) or Gal3-shRNA (green trace). Black trace is background staining. ( c ) ALDH activity was analyzed by flow cytometry. ALDEFLUOR activity in colon cancer and pancreatic cancer cells after infection with lentiviral particles for control-shRNA (blue trace) or Gal3-shRNA (green trace). Gray profile is background staining

    Journal: Cell Death & Disease

    Article Title: Cell surface galectin-3 defines a subset of chemoresistant gastrointestinal tumor-initiating cancer cells with heightened stem cell characteristics

    doi: 10.1038/cddis.2016.239

    Figure Lengend Snippet: Silencing of Gal3 shifts CSC subset to a Gal3 negative CSC subset. ( a ) Western blot analysis for comparison of total Gal3 in LiM6TR or DLD1TR colon cancer cells and L3.6pl or AsPC1 pancreatic cancer cells after infection with lentiviral particles for control-shRNA ( c ) or Gal3-shRNA (G). Each pair of control and Gal3 knockdown cells were run side by side on the same gel. Comparison of Gal3 expression between cell lines has been previously published ( b ) Flow cytometric analysis of colon cancer and pancreatic cancer cells after infection with lentiviral particles for control-shRNA (red trace) or Gal3-shRNA (green trace). Black trace is background staining. ( c ) ALDH activity was analyzed by flow cytometry. ALDEFLUOR activity in colon cancer and pancreatic cancer cells after infection with lentiviral particles for control-shRNA (blue trace) or Gal3-shRNA (green trace). Gray profile is background staining

    Article Snippet: To identify and isolate CSC, parental cells were collected in Versene solution (Invitrogen, Carlsbad, CA, USA, cat#15040), washed and blocked with flow cytometry buffer (Santa Cruz Biotechnology, Dallas, TX, USA), labeled with the following primary conjugated antibodies and their matched isotypes: anti-CD24-PE-Cy7(561646), anti-EpCAM-FITC (BD Bioscience, San Jose, CA, USA, 347197) anti-CD44-APC-eFluor 780(47044182), anti-CD166-PerCPeFluor 710(46166842), anti Gal3-PE(12-5301-83) (all from - eBioscience, San Diego, CA, USA).

    Techniques: Western Blot, Comparison, Infection, Control, shRNA, Knockdown, Expressing, Staining, Activity Assay, Flow Cytometry